px459 v2 plasmid (Addgene inc)

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Addgene inc px459 v2 plasmid
Px459 V2 Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmid+px459/HF-PX459+(V2)+(Plasmid+%23118632)/pmc13011211-62-7-9
Average 93 stars, based on 23 article reviews

px459 v2 plasmid - by Bioz Stars, 2026-09

93/100 stars

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Clone Assay:

Article Title: Temporal constraints on enhancer usage shape the regulation of limb gene transcription.
Article Snippet: .. Each sgRNA was cloned into the plasmid pX459 (Addgene, #48139), and 8 μg of each vector was used during mESCs transfection following the standard procedure for mESCs culture and genomic editing81. ..

Article Title: L1 insertion intermediates recombine with one another or with DNA breaks to form genome rearrangements
Article Snippet: .. The sgRNAs were cloned into the Cas9-expressing plasmid px459 (Addgene 62988, deposited by Dr. Feng Zhang ( )) by ligation of annealed oligonucleotides into Bbs I digested plasmid. ..

Article Title: Temporal constraints on enhancer usage shape the regulation of limb gene transcription
Article Snippet: .. Each sgRNA was cloned into the plasmid pX459 (Addgene, #48139), and 8 μg of each vector was used during mESCs transfection following the standard procedure for mESCs culture and genomic editing . ..

Plasmid Preparation:

Article Title: Siglec-15 is a putative receptor for porcine epidemic diarrhea virus infection
Article Snippet: The plasmid vectors used to overexpress the PEDV S1, S2, E, M, and N proteins (pLVX-S1-HA, pLVX-S2-HA, pLVX-E-Flag, pLVX-M-Flag, pLVX-N-Flag) were synthesized by Fuzhou Shangya Biotechnology Co., Ltd. (Fuzhou, China). .. The helper plasmids pCMV-VSV-G (#8454) and pSPAX2 for packaging lentivirus (#12260), the plasmid pX459 (#48139) for Siglec-15 knockout and the plasmid expressing TEV protease (pET28-MBP-super TEV protease) (#171782) were obtained from Addgene (Boston, MA, USA). ..

Article Title: Siglec-15 is a putative receptor for porcine epidemic diarrhea virus infection.
Article Snippet: The plasmid vectors used to overexpress the PEDV S1, S2, E, M, and N proteins (pLVX-S1-HA, pLVX-S2-HA, pLVXE-Flag, pLVX-M-Flag, pLVX-N-Flag) were synthesized by Fuzhou Shangya Biotechnology Co., Ltd. (Fuzhou, China). .. The helper plasmids pCMV-VSV-G (#8454) and pSPAX2 for packaging lentivirus (#12260), the plasmid pX459 (#48139) for Siglec-15 knockout and the plasmid expressing TEV protease (pET28-MBP-super TEV protease) (#171782) were obtained from Addgene (Boston, MA, USA). ..

Transfection:

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Expressing:

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Article Title: ARMC1 partitions between distinct complexes and assembles MIRO with MTFR to control mitochondrial distribution.
Article Snippet: MATERIALS AND METHODS Plasmids, oligonucleotides, siRNAs, and antibodies pX459 (Addgene, 62988) and guide RNA (gRNA)–AAVS1–T2 (Addgene, 41818), pOG44 (Invitrogen, V600520), pHDM- G (PlasmID, EvNO00061606), pHDM- HGPM2 (PlasmID, EvNO00061607), pHDM- tat1B (PlasmID, EvNO00061608), and pRC- CMV- rev1B (PlasmID, EvNO00061616) were obtained from the indicated suppliers. pDEST- hisMBP- AsCpf1- EC (Addgene, 79009) was modified to remove the maltose binding protein (MBP) sequence using standard molecular biology techniques.

Article Title: Termination sequence between an inducible promoter and ubiquitous chromatin opening element (UCOE) reduces gene expression leakage and silencing.
Article Snippet: These gRNAs were expressed using a plasmid based on the PX459 vector (Addgene#62988).

Article Title: Generation of a Mouse Model of Fuchs Endothelial Corneal Dystrophy by Knock-in of CTG Trinucleotide Repeat Expansion in the TCF4 Gene
Article Snippet: A plasmid expressing both Cas9 and single-guide RNA (sgRNA) targeting the Tcf4 exon 2 was constructed using the pX459 vector (no. 48139, Addgene, Cambridge, MA, USA) ( ).

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